exploring the protective effects of abrus precatorius in hepg2 and n-nitrosodiethylamine-induced hepatocellular carcinoma in swiss albino rats

نویسندگان

ramaswami kartik

pharmacognosy and ethnopharmacology division, national botanical research institute, rana pratap marg, lucknow-226 001, india chandana venkateshwara raoa

pharmacognosy and ethnopharmacology division, national botanical research institute, rana pratap marg, lucknow-226 001, india palpu pushpangadan

amity institute for herbal and biotech products development, thiruvananthapuram-695014, india sunil prakesh trivedi

environmental toxicology laboratory, department of zoology, university of lucknow, lucknow-226 007, india gaddam dayanand reddy

چکیده

this study was designed to explore the protective effects of abrus precatorius l. (leguminosae) (ap) in hepg2 cells and n-nitrosodiethylamine (ndea) induced hepatocellular carcinoma in swiss albino rats. the effects of aqueous/ethanolic (50%) extract of ap on hepatic markers, haematological and histopathological parameters, and antioxidant enzymes were evaluated in ndea (200 mg/kg and ccl4, 3 ml/kg body weight) induced experimental hepatocarcinogenesis in swiss albino rats. in addition, cytotoxicity of the extract and its effect on the expression on p53 were studied in human hepatoma cell line (hepg2). results obtained from cytotoxicity studies showed that the ap extract has strong cytotoxic effects on hepg2 cells. the expression of p53 was markedly increased and maintained at high level from 6-12 hr with 100 µg/ml of ap extract. a decrease in the mean and relative liver weights in ap extract treated group at a dose of 100 and 200 mg/kg was observed compared to the control group. it was also demonstrated that ap extract provided significant protection against hepatic lipid peroxidation and increased antioxidant enzymes’ activities such as superoxide dismutase, catalase, glutathione peroxidase, glutathione s-transferase and reduced glutathione levels. in a dose-dependent manner, the ap extract reduced the ndea-induced elevated levels of various hepatic markers such as serum glutamate pyruvate transaminase, serum glutamate oxaloacetate transaminase, alkaline phosphatase, total bilirubin and gamma glutamate transpeptidase. the haematological paremater viz. rbc, wbc and haemaglobin was restored upon treatment with ap extract at 100 and 200 mg/kg. histopathology of the liver was also carried out to mark the pathological changes in groups under study. the results of these studies demonstrate the protective effect of ap extract against ndea induced hepatocarcinogenesis in swiss albino rats and in hepg2 cell.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Exploring the Protective Effects of Abrus precatorius in HepG2 and N-Nitrosodiethylamine-Induced Hepatocellular Carcinoma in Swiss Albino Rats

      This study was designed to explore the protective effects of Abrus precatorius L. (Leguminosae) (AP) in HepG2 cells and N-nitrosodiethylamine (NDEA) induced hepatocellular carcinoma in Swiss albino rats. The effects of aqueous/ethanolic (50%) extract of AP on hepatic markers, haematological and histopathological parameters, and antioxidant enzymes were evaluated in NDEA ...

متن کامل

Protective effect of Genistein against N-nitrosodiethylamine (NDEA)-induced hepatotoxicity in Swiss albino rats

In the present study, we studied the effect of Genistein against the hepatotoxicity induced by N-nitrosodiethylamine (NDEA). NDEA is present in almost all kinds of food stuff and has been reported to be a hepatocarcinogen. The male rats were exposed to NDEA (0.1 mg/mL) dissolved in drinking water separately and along with 25, 50, 100 mg/mL of Genistein for 21 days. The activities of serum gluta...

متن کامل

Gender Disparity of N-nitrosodiethylamine Induced Hepatocellular Carcinoma in Wistar Albino Rats

Cancer is particularly alarming in developing countries like India where life expectancy is increasing rapidly. Over the past 25 years the incidence and mortality rates of Hepatocellular carcinoma (HCC) are expected to double over the next 10–20 years.HCC is a transformation process of normal liver cell into a cancerous cell which involves initiation, promotion and progression stages.HCC was in...

متن کامل

the effect of aqueous extract of garlic on formalin-induced pain in male rats

چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...

15 صفحه اول

Abrus precatorius Linn leaves in wistar rats

Abrus precatorius leaves have been used in traditional medicine for the treatment of a variety of diseases including cough, malaria and infertility in women. It has also been shown to have various useful pharmacological effects. In this study, oral acute toxicity of the aqueous, 70% methanol, Petroleum ether and Acetone extracts were investigated. The graphical method of Miller and Tainter was ...

متن کامل

Possible protective effects of resveratrol in hepatocellular carcinoma

Objective(s): Resveratrol (RSV) is a naturally occurring plant polyphenol with cardioprotective, neuroprotective, immunoregulatory, and anticancer properties and is biologically effective against various diseases. This study aimed to investigate the chemopreventive effect of different doses of RSV against hepatocellular carcinoma (HCC) induced by  diethylnitrosamine (D...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of pharmaceutical sciences

جلد ۶، شماره ۲، صفحات ۹۹-۱۱۴

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023